An HPLC Method for the Determination of Moxifloxacin in Breast Milk by Fluorimetric Detection with Precolumn Derivatization

Tekkeli S. E. K. , GAZIOGLU I. , KIZILTAS M. V.

ACTA CHROMATOGRAPHICA, vol.29, no.1, pp.57-65, 2017 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 1
  • Publication Date: 2017
  • Doi Number: 10.1556/1326.2017.29.1.4
  • Title of Journal : ACTA CHROMATOGRAPHICA
  • Page Numbers: pp.57-65
  • Keywords: breast milk, fluorescamine, fluorimetric detection, moxifloxacin, liquid chromatography, precolumn derivatization, PERFORMANCE LIQUID-CHROMATOGRAPHY, HUMAN PLASMA, FLUORESCENCE DETECTION, ULTRAVIOLET DETECTION, GATIFLOXACIN, LEVOFLOXACIN, TROVAFLOXACIN, SEPARATION, ASSAY


A new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min(-1). The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 mu g mL(-1). Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 mu g mL(-1), respectively. Intra-day and interday repeatabilities were less than 3.15%.