Akademik Veri Yönetim Sistemi
Elbay A. (Yürütücü), Dal A., Koçyiğit A., Hekimoğlu E. R., Sümbül B., Özman Z.
Yükseköğretim Kurumları Destekli Proje, BAP Araştırma Projesi, 2021 - 2021
Background: To investigate Quorum-Sensing
inhibition by furanone compounds in Pseudomonas aeruginosa keratitis rabbit
model.
Methods: Thirty adult New Zealand White
rabbits were used. Anesthetized rabbits were intrastromally injected with
Pseudomonas aeruginosa (P. aeruginosa). The rabbits were divided into six
groups: the control group (infected only with P. aeruginosa), group A (50 mg/mL
ceftazidime), group B (0.1 mg/mL furanone), group C (0.2 mg/mL furanone), group
D (0.3 mg/mL furanone), and group E (20% dimethyl sulfoxide). One drop of the
treatment was applied every hour for 3 days, starting 1-hour post-inoculation.
Rabbits were then sacrificed, and corneas were analysed clinically,
microbiologically, histologically, and biochemically. One-way analysis of variance was used for the mean comparison of
independent groups. The Least Significant Difference method was used as a
post-hoc test for pairwise comparisons.
Results: In all evaluations, the antibiotic
group (group A) showed the best therapeutic response. The slit-lamp examination
score of group C was significantly lowered than those compared of to the
control (p=0.009) and E groups (p=0.014). Histological evaluation showed that
inflammation is decreased in groups B, C, and D. Levels of cyclooxygenase-2
(COX-2), superoxide dismutase-1, and reactive oxygen species (ROS) were lowest
in the antibiotic-treated group, whereas the highest levels were detected in
the control group. Notably, the COX-2 levels in group B and ROS levels in
groups B and C were significantly lower than in control group. (p=0.045,
p=0.039 and p=0.045, respectively)
Conclusion: Furanone compounds may have minimal
therapeutic effects against Pseudomonas keratitis. Its therapeutic effect has
not been observed to be sufficient compared with that of antibiotics. Further
studies are needed to investigate their protective effects and mechanisms.