Rafıqı A. M. (Yürütücü)
Yükseköğretim Kurumları Destekli Proje, BAP Araştırma Projesi, 2018 - 2021
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Proje Türü:
Yükseköğretim Kurumları Destekli Proje
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Destek Programı:
BAP Araştırma Projesi
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Başlama Tarihi:
Kasım 2018
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Bitiş Tarihi:
Eylül 2021
Proje Özeti
The human gut harbours a complex ecosystem
composed of 1014 microbial cells. More than 1,500 different
bacterial species have already been associated with the human gut microbiota.
The close relationship between the host and microbial community causes
intestinal and extra-intestinal disorders. Over the last two decades,
development of next-generation sequencing (NGS) technology has significantly
increased our knowledge of gut microbiota. Tools permitting exhaustive analysis
of individual gut microbiota including a phylogenetic identification and
quantification are still under development. Most of these techniques are based
on the 16S ribosomal RNA (rRNA) gene sequence variations between different
species. 16S rRNA sequencing is a common, cost-effective, amplicon sequencing
method that can be analysed to determine the bacterial taxa and gives accurate
measures of taxonomic diversity. The aim of this project is to identify taxonomic classes of human microbes and to reveal
their association with diseases. Stool samples will be collected from
experimental and control groups, DNA will be isolated from the samples and next
generation sequencing of the obtained DNA samples based on the 16s rRNA gene
regions of the bacterial populations that constitute the microbiota. Statistical analysis will be performed to compare the results
between test and control groups. As a result, we will categorize microbiota of patients and healthy
individuals in Turkish populations. This
classification will allow identification of new interactions between diseases
and gut microbiome. These novel links will be used to develop therapeutic
targets and diseases and treatment approaches in the long term.